The coiled-coil domain is required for BECN1- and PIK3C3- binding and for autophagy.
Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes.
Cytoplasm. Endoplasmic reticulum. Note=Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
Forms a complex with BECN1, PIK3C3 and PIK3R4, but not with UVRAG, nor with KIAA0226/Rubicon. UVRAG and KIAA0831/Barkor form mutually exclusive complexes with BECN1 through direct competition.